ABSTRACT
AIM: To scrutinize the role of the Wnt/β-catenin signaling pathway in the epithelial-mesenchymal transition(EMT)of lens epithelial cells under hypoxic conditions, and to further analyze the effect of Dickkopf-1(DKK-1)expression on EMT of lens epithelial cells.METHODS: Human lens epithelial cells(HLEB3 cells)were propagated in vitro and then separated into two groups: one exposed to standard oxygen levels, added DMEM culture solution containing 10% FBS(normoxic group)and another subjected to low oxygen levels(hypoxic group). The hypoxic condition was emulated by applying a concentration of 100 μmol/L cobalt chloride(CoCl2)for 6, 12, 24, and 48h. The utilization of immunofluorescence staining enabled the detection of Wnt3a and DKK-1 expressions, along with the expression and localization of β-catenin protein in these groups. The expression of DKK-1 mRNA was discerned by quantitative real-time polymerase chain reaction(qRT-PCR).RESULTS: Immunofluorescence assays indicated an escalating trend in the Wnt3a and DKK-1 protein expression, which corresponded with the increasing duration of hypoxia. Likewise, an intensified nuclear accumulation of β-catenin protein was observed to be directly proportional to the length of hypoxia treatment. The qRT-PCR demonstrated that the difference in DKK-1 mRNA expression between the normoxic group and the group exposed to hypoxia for 6h was not statistically significant(P>0.05), whereas the DKK-1 mRNA expression of the 12, 24, and 48h hypoxia groups were significantly increased(P<0.001).CONCLUSION: Hypoxia can activate Wnt/β-catenin pathway in lens epithelial cells and induce the expression of DKK-1, thus regulating the Wnt/β-catenin pathway and affecting the EMT process.
ABSTRACT
Objective :To explore effects of Baduanjin combined medication on cardiac rehabilitation in patients with acute myocardial infarction (AMI).Methods :A total of 106 AMI cases treated in our hospital were enrolled .Ac-cording to random number table ,they were randomly and equally divided into medication group (received Tongxin-luo based on routine treatment ) and combined treatment group (received Baduanjin exercise based on medication group) ,both groups were treated for four weeks .Left ventricular end-diastolic volume (LVEDV) ,left ventricular end-systolic dimension (LVESd) ,left ventricular ejection fraction (LVEF) ,levels of tumor necrosis factor (TNF)-α ,high sensitive C reactive protein (hsCRP) ,interleukin (IL)-6 and N terminal pro brain natriuretic peptide (NT-proBNP) were observed and compared between two groups .Results : Total effective rate of combined treatment group was significantly higher than that of medication group (92.45% vs.75.47%,P＝0.017).Compared with be-fore treatment ,after treatment , there were significant rise in LVEDV and LVEF , and significant reductions in LVESd ,levels of hsCRP ,TNF-α ,IL-6 and NT-proBNP in two groups (P＜0.05 or ＜0.01).Compared with medi-cation group after treatment ,there were significant rise in LVEDV [ (153.58 ± 25.17) ml vs .(165.27 ± 25.46) ml] ,LVEF [(51.43 ± 4.28)% vs.(55.37 ± 4.26)% ] ,and significant reductions in LVESd [(33.46 ± 2.24) mm vs. (30.29 ± 2.32) mm] ,levels of hsCRP [ (4.54 ± 1.12) mg/L vs.(3.48 ± 1.05) mg/L] ,TNF-α [ (117.85 ± 15.46) ng/L vs.(96.59 ± 14.23) ng/L] ,IL-6 [ (14.38 ± 3.34) pg/ml vs.(10.67 ± 3.29) pg/ml] ,NT-proBNP [ (456.29 ± 53.76) ng/L vs.(368.49 ± 53.65) ng/L] in combined treatment group ,P＜0.05 or ＜0.01. Conclusion :Baduan-jin combined Tongxinluo capsule can effectively improve cardiac function ,reduce levels of inflammatory factors and improve therapeutic effect in patients with acute myocardial infarction .
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Abstract Mitral valve prolapse is a benign condition. Mitral regurgitation is only complicated in patients with severe mitral valve prolapse. Women with mitral valve prolapse in the absence of other cardiovascular disorders tolerate pregnancy well and do not develop remarkable cardiac complications. Nevertheless, serious complications of mitral valve prolapse, including arrhythmia, infective endocarditis and cerebral ischemic events, can be present in pregnancy. Debates remain with regard to the use of prophylactic antibiotics and β-blockers in the pregnant women with mitral valve prolapse. The prognosis of the pregnant patients might be closely related to the pathological and (or) functional changes of the mitral valve. Non-myxomatous mitral valve prolapse poses no or little obstetric risks in terms of pregnancy, labor and neonatal complications; whereas myxomatous mitral valve prolapse is a major etiology of valvular heart disease in women of childbearing age. In the pregnant patients with mitral valve prolapse progressing into major complications, surgical interventions are considered. Medicinal treatment of such patients with β-blockers should be a concern for the fetal safety.
Subject(s)
Humans , Female , Pregnancy , Pregnancy Complications, Cardiovascular/drug therapy , Mitral Valve Prolapse/drug therapy , Adrenergic beta-Agonists/therapeutic use , Pregnancy Complications, Cardiovascular/diagnosis , Prognosis , Pregnancy Outcome , Mitral Valve Prolapse/diagnosis , Mitral Valve Insufficiency/diagnosisABSTRACT
Objective To determine the significance of change of insulin-like growth factor-1(IGF-1) and study the effect of IGF-1 on expression of Bcl-2 and Bax.Methods Male Balb/c mice were randomly divided into control group,coxsackie B3(CVB3) infection group(infection group),CVB3 infection with IGF-1 treated group.Animals were killed at d7,d14.The immunohistochemical studies and medical imagine analysis system were performed to evaluate the expression of Bcl-2 and Bax in myocardial tissue.Serum IGF-1 concentrations were assayed by radioimmunoassay(RIA).Results In infection group,the concentrations of IGF-1 were more decreased than that of control group and the expression of Bcl-2 reduced and Bax increased(P